Enterohepatic circulation of bacterial chemotactic peptide in rats with experimental colitis

  • Hobson C
  • Butt T
  • Ferry D
 et al. 
  • 4


    Mendeley users who have this article in their library.
  • 68


    Citations of this article.


The association of hepatobiliary disorders with colonic inflammation is well recognized. Although the pathophysiology is obscure, increased permeation of toxic bacterial products across the inflamed gut to the portal circulation might be one mechanism. Potentially toxic metabolites include N-formylated chemotactic peptides that are produced by several species of intestinal bacteria and can be detected in colonic fluid in vivo. To investigate the metabolic fate of one of these low molecular weight proinflammatory peptides, N-formyl l-methionine l-leucine125I-l-tyrosine was introduced into colon loops of healthy rats (n = 10) and rats with experimental colitis (n = 15) induced by rectal instillation of 15% (vol/vol) acetic acid. Gut, liver, and blood radioactivity were monitored by external γ-counting and radioactivity in bile was measured by biliary catheter drainage into a well counter. Bile was processed by high-performance liquid chromatography to determine the amount of intact, bioactive peptide excreted over 3 h. After colonic instillation of 1 nmol of peptide, the mean (± SEM) biliary excretion of intact peptide was 6.4 ± 2.0 pmol in normal rats and 49.0 ± 20 pmol in rats with colitis (p < 0.01). An enterohepatic circulation of synthetic N-formyl l-methionine l-leucine l-tyrosine has been demonstrated in the rat. Experimental colitis was associated with an eightfold increase in biliary excretion of this proinflammatory bacterial peptide. Proinflammatory bacterial peptides synthesized by colonic bacteria could be important in the pathophysiology of colon inflammation and its frequently associated hepatobiliary complications. © 1988.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Christopher H. Hobson

  • Terence J. Butt

  • Dianne M. Ferry

  • June Hunter

  • Vinton S. Chadwick

  • Murray F. Broom

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free