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Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay

Kropinski A, Mazzocco A, Waddell T, Lingohr E, Johnson R ...see all
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Abstract

The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.

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Authors

  • Andrew M. Kropinski

  • Amanda Mazzocco

  • Thomas E. Waddell

  • Erika Lingohr

  • Roger P. Johnson

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