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Enumeration of bacteriophages by double agar overlay plaque assay

Kropinski A, Mazzocco A, Waddell T, Lingohr E, Johnson R ...see all

Bacteriophages: methods and protocols, Volume 1: isolation, characterization, and interactions, vol. 501, vol. 501 (2009) pp. 69-76 Published by Humana Press

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Abstract

This chapter describes a method for the generation of polyclonal antibodies against bacteriophages and how these may be assayed immunochemically and biologically.

Author-supplied keywords

  • direct plating
  • overlay technique
  • pfu
  • plaque
  • plaque morphology
  • plaque-forming unit

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Authors

  • Andrew M Kropinski

  • Amanda Mazzocco

  • Thomas E Waddell

  • Erika Lingohr

  • Roger P Johnson

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