Enumeration of bacteriophages by double agar overlay plaque assay.

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Abstract

The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.

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Kropinski, A. M., Mazzocco, A., Waddell, T. E., Lingohr, E., & Johnson, R. P. (2009). Enumeration of bacteriophages by double agar overlay plaque assay. Methods in Molecular Biology (Clifton, N.J.), 501, 69–76. https://doi.org/10.1007/978-1-60327-164-6_7

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