Enzymatic production of pectic oligosaccharides from polygalacturonic acid with commercial pectinase preparations

  • Combo A
  • Aguedo M
  • Goffin D
 et al. 
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Abstract

The present study investigates the individual efficiency of six commercial pectinase preparations (Endopolygalacturonase M2, Pectinase, Viscozyme L, Pectinex Ultra SP-L, Pectinase 62L and Macer8 FJ) in catalyzing the liberation of pectic oligosaccharides (POS) from polygalacturonic acid. On the basis of high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) analysis of the enzymatic hydrolysates, products release kinetics revealed a random cleavage pattern and an exo mode of cleavage for all the enzymes except for Endopolygalacturonase M2. All six enzymes generated oligoGalA with different degree of polymerization (DP); the quantitative composition of oligoGalA depended on the enzyme specificity and the time of enzymatic reaction. Endopolygalacturonase M2 was the best enzyme preparation for production of oligoGalA, with 18% (wt) of digalacturonic acid and 58% (wt) of trigalacturonic acid after 2 h of reaction. Concerning galacturonic acid production, Pectinase 62L was superior to the other enzyme preparations with 47% (wt) after 1 h of reaction. © 2011 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.

Author-supplied keywords

  • Degree of polymerization
  • HPAEC-PAD
  • Pectic oligosaccharides
  • Pectolytic enzymes
  • Polygalacturonic acid

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Authors

  • Agnan Marie Michel Combo

  • Mario Aguedo

  • Dorothée Goffin

  • Bernard Wathelet

  • Michel Paquot

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