Enzyme-linked immunosorbent assays for α-synuclein with species and multimeric state specificities.

  • Lee H
  • Bae E
  • Jang A
 et al. 
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Abstract

Abnormal intracellular deposition of aggregated α-synuclein is the characteristic feature of a number of neurological disorders, including Parkinson's disease (PD). Although α-synuclein is typically known as a cytosolic protein, a small amount is secreted by exocytosis in both monomeric and aggregated forms. The extracellular forms of α-synuclein in human body fluids, such as cerebrospinal fluid (CSF) and blood plasma, might be a diagnostic target for PD and related diseases. Here, we characterized a new set of monoclonal antibodies against α-synuclein, and using different combinations of antibodies, we established ELISA systems to specifically detect human α-synuclein, mouse and human α-synuclein together, and multimeric forms of α-synuclein in biological samples. By employing the Tyramide signal amplification method, the sensitivity of the assay was significantly improved to detect a concentration as low as ∼12.5 pg/ml. These assays might be useful tools for quantitative analysis of α-synuclein in various forms and with high sensitivity in diverse biological samples.

Author-supplied keywords

  • Adult
  • Animals
  • Antibodies, Monoclonal
  • Antibodies, Monoclonal: isolation & purification
  • Antibody Specificity
  • COS Cells
  • Cercopithecus aethiops
  • Enzyme-Linked Immunosorbent Assay
  • Enzyme-Linked Immunosorbent Assay: methods
  • Epitopes
  • Epitopes: immunology
  • Female
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Parkinson Disease
  • Parkinson Disease: blood
  • Parkinson Disease: cerebrospinal fluid
  • Parkinson Disease: immunology
  • Protein Multimerization
  • Protein Multimerization: immunology
  • Young Adult
  • alpha-Synuclein
  • alpha-Synuclein: immunology
  • alpha-Synuclein: isolation & purification
  • alpha-Synuclein: secretion

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Authors

  • He-Jin Lee

  • Eun-Jin Bae

  • Ara Jang

  • Dong-Hwan Ho

  • Eun-Duk Cho

  • Ji-Eun Suk

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