Estimating the incidence of entomopathogenic nematodes in soil by the use of bait insects

  • Nielsen O
  • Skovgaard I
  • Philipsen H
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Abstract

Soil samples were stored and baited in different ways in order to develop a bait method that could estimate the level of steinernematid nematodes in soil in a time-efficient way. The method was based on incidence (proportion of samples with a given species of Steinernema). Tenebrio molitor (Coleoptera) larvae were used as bait and it was concluded that: i) repeated baiting in a later period improved the bait result; ii) extension of the bait periods from 1-2 weeks was not necessary; iii) the precision of the estimated incidence did not improve by taking more than 60-80 samples; iv) the minimum time required to analyse one soil sample was 6 min.

Author-supplied keywords

  • Baiting
  • Sampling
  • Steinernema affine
  • Steinernema feltiae
  • Tenebrio molitor

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Authors

  • Otto Nielsen

  • Ib Michael Skovgaard

  • Holger Philipsen

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