Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk

Abstract

Rapid diagnostic tests are needed t o facilitate diagnosis and control of brucellosis. Sero - epidemiology of brucellosis is currently done by employing the Rose Bengal test (RBT). Also the world organization for animal health (OIE) has approved an indirect ELISA for testing serum and milk. Th e Immuno - Chromatographic brucellosis test (ICT) is a rapid, card - based immunochromatographic test for detection of antibodies directed against B. abortus antigens. To the best of our knowledge, ICT has not been used for the diagnosis of brucellosis in catt le yet . The objective of the study was to evaluate the performance of the ICT brucellosis test for the diagnosis of B. abortus in cattle sera versus RBT and ELISA as a gold standard, Also evaluation of ICT brucellosis test efficacy for detection of antibod ies against B. Abortus in milk samples. 94, 90.36 and 84.3% of sera samples were positive by RBT, ICT and ELISA respectively. The ICT had 94.44 % sensitivity and 100 % specificity versus RBT and ELISA as a gold standard. The kappa value between ICT and ELI SA was 0.76. Also ICT detected antibodies against B. abortus in serum and milk samples of 7 cows while a recently calved cow showed antibodies in the milk sample only. In conclusion, ICT provides a handy and accurate tool for the rapid detection of antibod ies to B. abortus in cattle sera and milk