Trizol extraction for polysaccharide-rich plant tissue was unsuitable for isolating total RNA from loquat fruit. CTAB-LiCl extraction was improved with pretreatment using washing buffer with 80% ethanol and 70% acetone. The RNA isolated by this protocol from different loquat fruit tissue at various development periods, was high in purity (A260/A280 ratio ranged from 1.81 to 1.99 and A260/A230 ratio was over 2.0), and high in yield.
CITATION STYLE
Ling, Z., Zhike, Z., Shunquan, L., Tingting, Z., & Xianghui, Y. (2013). Evaluation of Six Methods for Extraction of Total RNA from Loquat. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41(1), 313–316. https://doi.org/10.15835/nbha4118233
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