Evidence for intracellular spatial separation of hexokinases and fructokinases in tomato plants

  • Damari-Weissler H
  • Kandel-Kfir M
  • Gidoni D
 et al. 
  • 27


    Mendeley users who have this article in their library.
  • 37


    Citations of this article.


Four hexokinase (LeHXK1-4) and four fructokinase (LeFRK1-4) genes were identified in tomato plants. Previous GFP fusion studies indicate that the gene product of LeHXK3 is associated with the mitochondria while that of LeHXK4 is located within plastids. In this study we found that the enzyme encoded by the fructokinase gene LeFRK3 is also located within plastids. The presence of LeFrk3 enzyme in plastids raises the question of the origin of fructose in these organelles. The other three FRKs enzymes, LeFrk1&2&4, are located in the cytosol. Unlike LeFrk1&2&4, the two additional HXKs, LeHxk1&2, share a common membrane anchor domain and are associated with the mitochondria similar to LeHxk3. The difference in the locations of the cytoplasmic FRK and HXK isozymes suggests that glucose phosphorylation is confined to defined special intracellular localizations while fructose phosphorylation is less confined.

Author-supplied keywords

  • Fructokinase
  • GFP
  • Hexokinase
  • Hexose phosphorylation
  • Intracellular localization
  • Tomato (Lycopersicon esculentum)

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Hila Damari-Weissler

  • Michal Kandel-Kfir

  • David Gidoni

  • Anahit Mett

  • Eddy Belausov

  • David Granot

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free