Exploiting glycation to stiffen and strengthen tissue equivalents for tissue engineering

  • Girton T
  • Oegema T
  • Tranquillo R
  • 94

    Readers

    Mendeley users who have this article in their library.
  • 147

    Citations

    Citations of this article.

Abstract

Glycation, the nonenzymatic crosslinking of proteins by reducing sugars, is known to cause stiffening of soft tissues over a lifetime, particularly in diabetics. We show here that glycation due to elevated glucose and ribose concentrations in cell culture medium can be exploited in a matter of a few weeks of incubation to stiffen and strengthen tissue equivalents and to increase their resistance to collagenolytic degradation, all without loss of cell viability. Glycated tissue equivalents did not elicit inflammation or induce calcification upon subcutaneous implantation; rather, they were permissive to host integration and remodeling. Thus a pathological process might be used in a targeted way in tissue engineering to fabricate tissue equivalents with the required mechanical properties and desired resorption rate upon implantation.

Author-supplied keywords

  • Glycation
  • Mechanical stiffening
  • Mechanical strengthening
  • Tissue engineering
  • Tissue equivalent

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • T. S. Girton

  • T. R. Oegema

  • R. T. Tranquillo

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free