Exploiting the yeast Saccharomyces cerevisiae for the study of the organization and evolution of complex genomes

  • Kouprina N
  • Larionov V
  • 32

    Readers

    Mendeley users who have this article in their library.
  • 14

    Citations

    Citations of this article.

Abstract

Yeast artificial chromosome (YAC) cloning systems have advanced the analysis of complex genomes considerably. They permit the cloning of larger fragments than do bacterial artificial chromosome systems, and the cloned material is more easily modified. We recently developed a novel YAC cloning system called transformation-associated recombination (TAR) cloning. Using in vivo recombination in yeast, TAR cloning selectively isolates, as circular YACs, desired chromosome segments or entire genes from complex genomes. The ability to do that without constructing a representative genomic library of random clones greatly facilitates analysis of gene function and its role in disease. In this review, we summarize how recombinational cloning techniques have advanced the study of complex genome organization, gene expression, and comparative genomics. © 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Author-supplied keywords

  • Complex genome
  • Gene isolation
  • Transformation-associated recombination cloning
  • Yeast artificial chromosome

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Natalay Kouprina

  • Vladimir Larionov

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free