Journal article

Expression of Cdk5 and its activators in NT2 cells during neuronal differentiation

Fu W, Wang J, Ip N ...see all

Journal of Neurochemistry, vol. 81, issue 3 (2002) pp. 646-654

  • 10

    Readers

    Mendeley users who have this article in their library.
  • 21

    Citations

    Citations of this article.
Sign in to save reference

Abstract

We have recently developed a rapid protocol involving NT2 cell aggregation and treatment with retinoic acid (RA) to produce terminally differentiated CNS neurons. As a first step to explore the functional roles of cell-cycle regulatory proteins in the process of neuronal differentiation, the expression profiles of cyclin-dependent kinases (Cdks) and their regulators were examined in NT2 cells following treatment with RA. One of the Cdks, Cdk5, has been demonstrated to affect the process of neuronal differentiation and suggested to play an important role in development of the nervous system. We found that the expression of Cdk5 was gradually increased, while its activators (p35 and p39) as well as Cdk5 kinase activity were induced in NT2 cells during the process of neuronal differentiation. Moreover, both p35 and p39 were localized along the axons and varicosity-like structures of differentiated NT2 neurons. Taken together, our results demonstrated that NT2 cells provide a good in vitro model system to examine signaling pathways involved in the regulation of Cdk5 activators and to elucidate the functional roles of Cdk5 in neuronal differentiation.

Author-supplied keywords

  • Cdk5
  • Cell cycle
  • Cyclin-dependent kinase
  • Embryonal carcinoma
  • NT2
  • Neuronal differentiation

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text

Authors

  • Wing Yu Fu

  • Jerry H. Wang

  • Nancy Y. Ip

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free