Expression of a chitinase gene and lysis of the host cell wall during Chlorella virus CVK2 infection

  • Hiramatsu S
  • Ishihara M
  • Fujie M
 et al. 
  • 31


    Mendeley users who have this article in their library.
  • 34


    Citations of this article.


A chitinase gene (vChti-1) encoded by the Chlorella virus CVK2 was cloned and characterized. The vChti-1 open reading frame consisted of 2508 bp corresponding to 836 amino acid residues. The predicted amino acid sequence contained two sets of a family 18 catalytic domain that is responsible for chitinase activity. Northern blot analysis revealed that the vChti-1 gene was expressed in virus-infected Chlorella cells late in infection, when a single transcript of about 2.5 kb appeared at 120 min postinfection. This result was confirmed by Western blotting with a specific anti-vChti-1 protein antibody; a protein of about 94 kDa was detected specifically beginning at 240 min postinfection and was present until cell lysis. The protein was not incorporated into viral particles but remained in the medium after cell lysis. The vChti-1 protein produced in virus-infected cells showed chitinase activity on zymogram assays.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Shingo Hiramatsu

  • Masako Ishihara

  • Makoto Fujie

  • Shoji Usami

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free