Expression of the murine complement regulatory protein crry by glial cells and neurons.

  • Davoust N
  • Nataf S
  • Holers V
 et al. 
  • 8


    Mendeley users who have this article in their library.
  • N/A


    Citations of this article.


The expression of the murine complement regulatory protein, Crry, in the CNS remains largely unexplored. In this study, we examined murine astrocytes and microglia purified from neonatal brain and sections of adult murine brain for the expression of Crry. Using RT-PCR, immunohistochemistry, in situ hybridization, flow cytometry, and Western blot analysis, we demonstrated that astrocytes and microglia express Crry protein and RNA. Crry expression is greater on microglia than astrocytes and, as determined by Western blot analysis, each cell type expresses a Crry protein of different molecular weight. Interestingly, neuronal expression of Crry was seen only at the RNA level. These data demonstrate Crry expression by astrocytes, microglia, and neurons in the murine CNS and suggest that Crry may play an important role in protecting the CNS against complement-mediated damage.

Author-supplied keywords

  • Animals
  • Astrocytes
  • Astrocytes: metabolism
  • Blotting, Western
  • Complement Activation
  • Flow Cytometry
  • Gene Expression Regulation
  • In Situ Hybridization
  • Mice
  • Mice, Inbred C57BL
  • Microglia
  • Microglia: metabolism
  • Nerve Tissue Proteins
  • Nerve Tissue Proteins: biosynthesis
  • Nerve Tissue Proteins: genetics
  • Neurons
  • Neurons: metabolism
  • RNA, Messenger
  • RNA, Messenger: biosynthesis
  • RNA, Messenger: genetics
  • Receptors, Complement
  • Receptors, Complement: biosynthesis
  • Receptors, Complement: genetics
  • Reverse Transcriptase Polymerase Chain Reaction

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • N Davoust

  • S Nataf

  • V M Holers

  • S R Barnum

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free