Expression and secretion of a functional scorpion insecticidal toxin in cultured mouse cells

  • Dee A
  • Belagaje R
  • Ward K
 et al. 
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Abstract

We have expressed a synthetic gene encoding the insecticidal neurotoxin of scorpion Androctonus australis (AaIT) in NIH/3T3 mouse fibroblast cells under the transcriptional control of a murine retroviral long terminal repeat. The secretion of the toxin into the culture medium was directed by the signal peptide of human interleukin-2. The recombinant AaIT produced was selectively toxic to yellow-fever mosquito larvae and harmless to mice.

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Authors

  • Albert Dee

  • Rama M. Belagaje

  • Karen Ward

  • Eddie Chio

  • Mei Huei T. Lai

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