Expression of a single-copy hsp-16.2 reporter predicts life span

  • Mendenhall A
  • Tedesco P
  • Taylor L
 et al. 
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The level of green fluorescent protein expression from an hsp-16.2-based transcriptional reporter predicts life span and thermotolerance in Caenorhabditis elegans. The initial report used a high-copy number reporter integrated into chromosome IV. There was concern that the life-span prediction power of this reporter was not attributable solely to hsp-16.2 output. Specifically, prediction power could stem from disruption of some critical piece of chromatin on chromosome IV by the gpIs1 insertion, a linked mutation from the process used to create the reporter, or from an artifact of transgene regulation (multicopy transgenes are subject to regulation by C elegans chromatin surveillance machinery). Here we determine if the ability to predict life span and thermotolerance is specific to the gpIs1 insertion or a general property of hsp-16.2-based reporters. New single-copy hsp-16.2-based reporters predict life span and thermotolerance. We conclude that prediction power of hsp-16.2-based transcriptional reporters is not an artifact of any specific transgene configuration or chromatin surveillance mechanism.

Author-supplied keywords

  • Aging
  • Biomarker of life span
  • Heat shock
  • Longevity
  • Transgene

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  • Alexander R. Mendenhall

  • Patricia M. Tedesco

  • Larry D. Taylor

  • Anita Lowe

  • James R. Cypser

  • Thomas E. Johnson

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