Extraction of shikimic acid from chinese star anise using flash column chromatography on a molecularly-imprinted polymer column

  • Xue M
  • Wang Y
  • Meng Z
 et al. 
  • 8

    Readers

    Mendeley users who have this article in their library.
  • 7

    Citations

    Citations of this article.

Abstract

Flash column chromatography prepacked with molecularly imprinted polymers (MIPs) has been successfully used for the extraction of shikimic acid from Chinese star anise. The shikimic acid imprinted polymer with an adsorption capacity of 21.94 mu mol g(-1) dry polymer and imprinting factor of 4.2 was prepared using the dispersion polymerization method. The capacity of the MIP-Flash column was determined as 23.3mg g(-1) using a frontal chromatography method when 10m mol L-1 shikimic acid was eluted through the MIP-flash column at 1.7mL min(-1). The crude extract of Chinese star anise loaded on the column was eluted with ethanol/water (40/60, v/v) to remove the impurities, after elution with methanol/water/acetic acid (32/48/20, v/v/v), purified shikimic acid (>95%) was obtained. The MIP-flash column can be reused for at least 4 rounds with capacity lost less than 15%. It was demonstrated that, compared with HPLC, flash column chromatography prepacked with MIPs is a more rapid, feasible, and economical method for the extraction of active ingredients from plants on a large scale based on the technology of molecular imprinting.

Author-supplied keywords

  • HPLC
  • molecularly imprinted technology
  • natural product
  • polymer chemistry
  • proteomics
  • separation science and technology

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text

Authors

  • Min Xue

  • Yu Wang

  • Zihui Meng

  • Weibing Zhang

  • Yin Wu

  • Shikuan Jiang

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free