Fabrication and Characterization of HER2 Cell Receptor-Targeted Indocyanine Green-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles

Abstract

Introduction: The aim of this study is to fabricate and characterize human epidermal growth factor receptor 2 (HER2)-targeted indocyanine green (ICG)-loaded poly (lactic-co-glycolic acid (PLGA) nanoparticles (HIPNPs). Methods: The HIPNPs were fabricated by a modified emulsification in association with solvent evaporation approach. The size and surface charge of the manufactured nanoparticles were determined by dynamic light scattering technique. The morphology of the HIPNPs was detected by SEM. The activity of surface anchored anti-HER2 antibodies was detected by spectro fluorometry and fluorescent microscopy. The encapsulation rate of ICG, percentage of ICG content, and the degradation efficiencies of entrapped ICG under different temperatures were measured through UVVis spectrometry.