Fasciola hepatica procathepsin L3 protein expressed by a baculovirus recombinant can partly protect rats against fasciolosis

  • Reszka N
  • Cornelissen J
  • Harmsen M
 et al. 
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Fasciola hepatica juveniles express immunodominant cathepsin L proteins, which are mainly found in their immature, procathepsin form. A gene encoding such a procathepsin L (FheCL3) was expressed by a baculovirus recombinant and by Saccharomyces cerevisiae. The glycosylated FheCL3 proteins obtained by both systems were used in a vaccination/challenge experiment in rats. Both antigens evoked similar antibody responses, but only the baculovirus expressed FheCL3 caused a significant protection against the number of liver flukes (52% protection, P = 0.01), whereas the S. cerevisiae expressed FheCL3 did not. In a second experiment in rats, deglycosylated versions of both antigens were used, but this did not improve their efficacies. © 2004 Elsevier Ltd. All rights reserved.

Author-supplied keywords

  • Fasciola hepatica
  • Procathepsin L3
  • Vaccine

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  • Natalia Reszka

  • Jan B.W.J. Cornelissen

  • Michiel M. Harmsen

  • Krystyna Bieńkowska-Szewczyk

  • Joop De Bree

  • Wim J. Boersma

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