Fast haptoglobin phenotyping based on microchip electrophoresis

  • Huang B
  • Huang C
  • Liu P
 et al. 
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Abstract

A new and fast method for haptoglobin phenotyping was developed based on microchip electrophoresis with laser induced fluorescence detection. Haptoglobin phenotypes 1-1 and 2-2 were labeled with fluorescein isothiocyanate. The analyses were performed on glass microchip which was simply treated with sodium dodecyl sulfate. After the optimization of the separation conditions, Hp 1-1 and Hp 2-2 could be differentiated in 150 s and the detection limits for Hp 1-1 and Hp 2-2 were 0.39 and 0.62 μg/mL, respectively. Finally, the method was applied to human serum samples from healthy people and liver cancer patients. A decrease in Hp concentration for liver cancer patients was confirmed. Featuring high efficiency, speed, simplicity, the method reveals great potentials for the diagnosis of diseases and proteome research. © 2011 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • Haptoglobin phenotype
  • Laser-induced fluorescence detection
  • Microchip electrophoresis

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Authors

  • Bingrong Huang

  • Changgang Huang

  • Pingping Liu

  • Fangfang Wang

  • Na Na

  • Jin Ouyang

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