Fertilization efficiency of cryopreserved sperm from striped catfish, Pangasius hypophthalmus (Sauvage)

  • Kwantong S
  • Bart A
  • 17


    Mendeley users who have this article in their library.
  • 8


    Citations of this article.


The fertilization efficiency of cryopreserved sperm was compared with fresh sperm from striped catfish, Pangasius hypophthalmus. Of the two sets of experiments carried out, the first compared four sperm doses using fresh sperm and fresh eggs. The second experiment compared six concentrations of cryopreserved sperm ranging from 6.94 x 107 to 6.94 x 1010 to fertilize100 eggs per batch. Fertilization, hatch and survival rates were compared between cryopreserved and fresh sperm. The highest fertilization rate (53.75 x 1.62 %) was achieved with a sperm dose of 6.94 x 108. Increasing the sperm dose to 3.47 x 109 did not increase the fertilization rate, indicating that the optimum sperm:egg ratio lies between 6.94 x 106 and 3.47 x 107 sperm per egg. Both high- est (6.94 x 1010) and the lowest (6.94 x 107) sperm doses resulted in lower fertilization rates (2.04% and 16.90% respectively). No significant differences were found among four fresh sperm doses compared. Mean hatch and survival rates resulting from fresh and cryopreserved sperm were similar. The experiment shows that while only 1.89 x 106 fresh spermatozoa was required to fertilize a fresh egg, 6.94 x 106 (or 3.67 times more) cryopreserved sperm was required to achieve the same level of fertilization. This provides important information for making decision to cryopreserve sperm for commercial and/or conservation purposes.

Author-supplied keywords

  • Cryopreservation
  • Fertilization
  • Pangasius hypophthalmus
  • Sperm

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free