Field Demonstration of Successful Bioaugmentation To Achieve Dechlorination of Tetrachloroethene To Ethene

  • Major D
  • Cox E
  • Dworatzek S
 et al. 
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A laboratory microcosm study and a pilot scale field test were conducted to evaluate biostimulation and bioaugmentation to dechlorinate tetrachloroethene (PCE) to ethene at Kelly Air Force Base. The site groundwater contained about 1 mg/L of PCE and lower amounts of trichloroethene (TCE) and cis-1,2-dichloroethene (cDCE). Laboratory microcosms inoculated with soil and groundwater from the site exhibited partial dechlorination of TCE to cDCE when amended with lactate or methanol. Following the addition of a dechlorinating enrichment culture, KB-1, the chlorinated ethenes in the microcosms were completely converted to ethene. The KB-1 culture is a natural dechlorinating microbial consortium that contains phylogenetic relatives of Dehalococcoides ethenogenes. The ability of KB-1 to stimulate biodegradation of chlorinated ethenes in situ was explored using a closed loop recirculation cell with a pore volume of approximately 64 000 L. The pilot test area (PTA) groundwater was first amended with methanol and ac...

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  • David W. Major

  • Evan E. Cox

  • Sandra M. Dworatzek

  • Edwin R. Hendrickson

  • Mark G. Starr

  • Jo Ann O A N N Payne

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