Fluorescence detection of glycoproteins in gels and on electroblots.

  • Patton W
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Abstract

Glycosylation of proteins is a dynamic post-translational modification commonly found in eukaryotes, changing with development, differentiation, and disease state. Identification of glycoproteins is important for completely characterizing the population of proteins found at a given point in time. This unit contains protocols for identifying glycoproteins in general using a fluorescence based periodate/Schiff base method and those containing specific carbohydrates using a lectin-based fluorescent method.

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Authors

  • Wayne F Patton

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