Fluorescence labeling of oligosaccharides useful in the determination of molecular interactions.

  • Sugahara D
  • Amano J
  • Irimura T
  • 13


    Mendeley users who have this article in their library.
  • 9


    Citations of this article.


A simple method to label oligosaccharides with a multifunctional fluorescent group was developed. Oligosaccharides were quantitatively labeled at their reducing termini with pyrene butanoic acid hydrazide. The pyrene-labeled oligosaccharides were successfully applied to fluorescence polarization measurements and ELISA at picomole quantity, which was not previously reached by other procedures. This labeling method should prove to be useful in a variety of aspects in glycobiology.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Daisuke Sugahara

  • Junko Amano

  • Tatsuro Irimura

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free