Fluorescence molecular tomography (FMT), an in vivo noninvasive imaging technology, can provide localization and quantification information for deep fluorophores. Light at wavelengths in the near-infrared (NIR-I) window from 650 nm to 950 nm has conventionally been chosen for FMT. In this study, we introduced longer NIR wavelengths within the 1100 nm to 1400 nm range, known as the "second NIR spectral window" (NIR-II). A singular-value analysis method was used to demonstrate the utility and advantages of using the NIR-II for FMT, and experiments showed an improvement in the spatial resolution in phantom studies.
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