This chapter describes the use of microscope-based fluorescence recovery after photobleaching (FRAP). To quantify the dynamics of proteins within a subcellular compartment, we first outline the general aspects of FRAP experiments and then provide a detailed protocol of how to measure and analyse the most important parameters of FRAP experiments such as mobile fraction and half-time of recovery.
CITATION STYLE
Carisey, A., Stroud, M., Tsang, R., & Ballestrem, C. (2011). Fluorescence Recovery After Photobleaching. In Methods in Molecular Biology (Vol. 769, pp. 387–402). Humana Press Inc. https://doi.org/10.1007/978-1-61779-207-6_26
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