Fluorescent labeling of peptides on solid phase

  • Katritzky A
  • Yoshioka M
  • Narindoshvili T
 et al. 
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Abstract

N(alpha)-Fmoc-N(epsilon)-[(7-methoxycoumarin-4-yl)acetyl]-L-lysine (N(alpha)-Fmoc-L-Lys(Mca)-OH) 3 is conveniently prepared by benzotriazole methodology (52% over two steps). N-Acylbenzotriazoles Mca-Bt 2, N(alpha)-Fmoc-L-Lys(Mca)-Bt 4, coumarin-3-ylcarbonyl (Cc)-Bt 5, N(alpha)-Fmoc-L-Lys(Cc)-Bt 7 and N(alpha)-(Cc)-L-Lys(Fmoc)-Bt 9 enable the efficient microwave enhanced solid-phase fluorescent labeling of peptides.

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Authors

  • Alan R. Katritzky

  • Megumi Yoshioka

  • Tamari Narindoshvili

  • Alfred Chung

  • Jodie V. Johnson

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