Full genomic amplification and subtyping of influenza a virus using a single set of universal primers

  • Inoue E
  • Wang X
  • Osawa Y
 et al. 
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Abstract

Influenza A virus has eight-segmented RNA molecules as a genome and, among all strains of the virus, both ends of each segment have 13 and 12 nucleotide sequences conserved. In the present study, a simple RT-PCR method to amplify all eight segments of the virus and determine the HA and NA subtype using a single primer set based on the conserved terminal sequences has been established. This method is also capable of detecting subgenomic defective interfering RNA of the influenza A virus. Since the primers used here cope with each and every RNA segment of influenza A virus, this simple RT-PCR method is valuable not only for cloning each gene of the virus, but also for identifying subtypes, including subtypes other than 16 HA and 9 NA subtypes.

Author-supplied keywords

  • Genomic amplification
  • Influenza A virus
  • Single primer set
  • Subtyping

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