Functional characterization of FaCCD1: A carotenoid cleavage dioxygenase from strawberry involved in lutein degradation during fruit ripening

  • García-Limones C
  • Schnäbele K
  • Blanco-Portales R
 et al. 
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A gene encoding a carotenoid cleavage dioxygenase class 1 enzyme (FaCCD1) was identified among a strawberry fruit expressed sequence tag collection. The full-length cDNA was isolated, and the expression profiles along fruit receptacle development and ripening, determined by quantitative real time polymerase chain reaction, showed that FaCCD1 is a ripening-related gene that reaches its maximal level of expression in the red fully ripe stage. FaCCD1 was expressed in Escherichia coli, and the products formed by the recombinant protein through oxidative cleavage of carotenoids were identified by liquid chromatography−mass spectrometry and gas chromatography−mass spectrometry analyses. The FaCCD1 protein cleaves zeaxanthin, lutein, and β-apo-8′-carotenal in vitro. Although β-carotene is not a good substrate for FaCCD1 in vitro, the expression of FaCCD1 in an engineered carotenoid-producing E. coli strain caused the degradation of β-carotene in vivo. Additionally, the carotenoid profile in strawberry was analyz...

Author-supplied keywords

  • CCD1
  • Carotenoids
  • Fragaria x ananassa lutein
  • Gene expression
  • Ripening
  • Strawberry

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  • Carmen García-Limones

  • Kerstin Schnäbele

  • Rosario Blanco-Portales

  • M. Luz Bellido

  • Jose Luis Caballero

  • Wilfried Schwab

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