Functionally interacting telomerase RNAs in the yeast telomerase complex

  • Prescott J
  • Blackburn E
  • 40

    Readers

    Mendeley users who have this article in their library.
  • 139

    Citations

    Citations of this article.

Abstract

The ribonucleoprotein (RNP) enzyme telomerase from Saccharomyces cerevisiae adds telomeric DNA to chromosomal ends in short increments both in vivo and in vitro. Whether or not telomerase functions as a multimer has not been addressed previously. Here we show, first, that following polymerization, the telomerase RNP remains stably bound to its telomeric oligonucleotide reaction product. We then exploit this finding and a previously reported mutant telomerase RNA to demonstrate that, unexpectedly, the S. cerevisiae telomerase complex contains at least two functionally interacting RNA molecules that both act as templates for DNA polymerization. Here, functional telomerase contains at least two active sites.

Author-supplied keywords

  • Dimer
  • Nondissociative
  • Nonprocessive
  • Telomerase
  • Yeast

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text

Authors

  • John Prescott

  • Elizabeth H. Blackburn

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free