Gc Protein-derived Macrophage Activating Factor (GcMAF): Isoelectric Focusing Pattern and Tumoricidal Activity

  • Mohamad S
  • Nagasawa H
  • Sasaki H
 et al. 
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Abstract

BACKGROUND: Gc protein is the precursor for Gc protein-derived macrophage activating factor (GcMAF), with three phenotypes: Gc1f, Gc1s and Gc2, based on its electrophoretic mobility. The difference in electrophoretic mobility is because of the difference in its posttranslational sugar moiety composition. MATERIALS AND METHODS: We compared the difference between Gc protein and GcMAF electrophoretic mobility using the isoelectric focusing (IEF) method. The tumoricidal activity of GcMAF-treated macrophage was evaluated after coculture with L-929 cell. The tumoricidal mechanism was investigated using TNF bioassay and nitric oxide (NO) release. RESULTS: The difference in Gc protein and GcMAF electrophoretic mobility was detected. The tumoricidal activity of GcMAF-treated macrophage was detected, but no release of TNF and NO was detected. CONCLUSION: The difference of isoelectric focusing mobility in Gc protein and GcMAF would be useful to develop a GcMAF detection method. GcMAF increased macrophage tumoricidal activity but TNF and NO release were not involved in the mechanism

Author-supplied keywords

  • Gc protein-derived macrophage activating factor (GcMAF)
  • Isoelectric focusing (IEF)
  • Nitric oxide (NO)
  • Tumor necrosis factor (TNF)
  • Tumoricidal

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Authors

  • Saharuddin Bin Mohamad

  • Hideko Nagasawa

  • Hideyuki Sasaki

  • Yoshihiro Uto

  • Yoshinori Nakagawa

  • Ken Kawashima

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