Geneious! Simplified Genome Skimming Methods for Phylogenetic Systematic Studies: A Case Study in Oreocarya (Boraginaceae) Ap Applications ons in in Pl Plant t Scien Sciences ces

  • Ripma L
  • Simpson M
  • Hasenstab-Lehman K
  • 2

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Abstract

BioOne (www.bioone.org) is a nonprofit, online aggregation of core research in the biological, ecological, and environmental sciences. BioOne provides a sustainable online platform for over 170 journals and books published by nonprofit societies, associations, museums, institutions, and presses. Your use of this PDF, the BioOne Web site, and all posted and associated content indicates your acceptance of BioOne's Terms of Use, available at www.bioone.org/page/terms_of_use. The power of next-generation sequencing (NGS) is trans-forming the study of nonmodel plant taxa (Soltis et al., 2013). Sweeping statements about the utility of NGS to answer previ-ously intractable questions are commonplace in systematics journals. The initial bioinformatic hurdle and the fact that NGS technology can be used in different ways (see review by Godden et al., 2012 ; Soltis et al., 2013) inhibit many systematists from beginning studies. Briefl y, many NGS library preparation meth-ods rely on genome reduction, including targeting the tran-scriptome (e.g., Wen et al., 2013), nuclear loci (e.g., Weitemier et al., 2014), or the plastome (e.g., Stull et al., 2013). Reduction techniques capturing large numbers of nuclear loci require baseline genomic knowledge (see review by Cronn et al., 2012). In contrast, systematists can use the NGS genome skimming method (Straub et al., 2012) to assemble the high-copy frac-tion of total genomic DNA (gDNA) into the nuclear ribosomal

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Authors

  • Lee A Ripma

  • Michael G Simpson

  • Kristen Hasenstab-Lehman

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