Genetic analysis of RFLPs, GATA microsatellites and RAPDs in a cross between L. esculentum and L. pimpinellifolium

  • Grandillo S
  • Tanksley S
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Abstract

A population of 257 BC1 plants was developed from a cross between an elite processing line of tomato (Lycopersicon esculentum cv. M82-1-7) and the closely related wild species L. pimpinellifolium (LA1589). The population was used to construct a genetic linkage map suitable for quantitative trait locus (QTL) analysis to be conducted in different backcross generations. The map comprises 115 RFLP, 3 RAPD and 2 morphological markers that span 1279 cM of the tomato genome with an average distance between markers of 10.7 cM. This map is comparable in length to that of the high-density RFLP map derived from a L. esculentum X L. pennellii F2 population. The order of the markers in the two maps is also in good agreement, however, there are considerable differences in the distribution of recombination along the chromosomes. The segregation of six GATA-containing loci and 47 RAPD markers was also analysed in subsets of the population. All of the microsatellite loci and 35 (75%) of the RAPDs mapped to clusters associated with centromeric regions

Author-supplied keywords

  • GATA microsatellites
  • L. pimpinellifolium
  • Mapping
  • RAPDs
  • RFLPs

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Authors

  • S. Grandillo

  • S. D. Tanksley

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