Genetic diversity of MHC class i loci in six non-model frogs is shaped by positive selection and gene duplication

  • Kiemnec-Tyburczy K
  • Richmond J
  • Savage A
 et al. 
  • 54


    Mendeley users who have this article in their library.
  • 24


    Citations of this article.


Comparative studies of major histocompatibility complex (MHC) genes across vertebrate species can reveal the evolutionary processes that shape the structure and function of immune regulatory proteins. In this study, we characterized MHC class I sequences from six frog species representing three anuran families (Hylidae, Centrolenidae and Ranidae). Using cDNA from our focal species, we amplified a total of 79 unique sequences spanning exons 2-4 that encode the extracellular domains of the functional alpha chain protein. We compared intra- and interspecific nucleotide and amino-acid divergence, tested for recombination, and identified codon sites under selection by estimating the rate of non-synonymous to synonymous substitutions with multiple codon-based maximum likelihood methods. We determined that positive (diversifying) selection was acting on specific amino-acid sites located within the domains that bind pathogen-derived peptides. We also found significant signals of recombination across the physical distance of the genes. Finally, we determined that all the six species expressed two or three putative classical class I loci, in contrast to the single locus condition of Xenopus laevis. Our results suggest that MHC evolution in anurans is a dynamic process and that variation in numbers of loci and genetic diversity can exist among taxa. Thus, the accumulation of genetic data for more species will be useful in further characterizing the relative importance of processes such as selection, recombination and gene duplication in shaping MHC loci among amphibian lineages.

Author-supplied keywords

  • anura
  • immunogenetics
  • major histocompatibility complex
  • molecular evolution

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • K. M. Kiemnec-Tyburczy

  • J. Q. Richmond

  • A. E. Savage

  • K. R. Lips

  • K. R. Zamudio

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free