Genetic engineering of Trichoderma to produce strains with novel cellulase profiles

  • Harkki A
  • Mäntylä A
  • Penttilä M
 et al. 
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Abstract

Genetic engineering has been used to modify the proportion of different cellulases produced by a hypercellulolytic Trichoderma reesei mutant strain. A general expression vector, pAMH110, containing the promoter and terminator sequences of the strongly expressed main cellobiohydrolase 1 (cbh1) gene was used to overexpress a cDNA coding for EGI, the major endoglucanase (1,4,β-d-glucan glucanohydrolase, EC 3.2.1.4). An in vitro modified cbh1 cDNA, incapable of coding for active enzyme, was used to inactivate the major cellobiohydrolase (1,4-β-d-glucan cellobiohydrolase, EC 3.2.1.91) gene. In this way, new strains producing elevated amounts of the specific endoglucanase 1 (EGI) and/or lacking the major cellobiohydrolase (CBHI) were produced, and these have been further characterized. © 1991.

Author-supplied keywords

  • Trichoderma reesei
  • cellulases
  • gene inactivation
  • hypercellulolytic mutant
  • overexpression
  • strain improvement

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Authors

  • Anu Harkki

  • Arja Mäntylä

  • Merja Penttilä

  • Susanna Muttilainen

  • Rolf Bühler

  • Pirkko Suominen

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