The genetic regulation of hydroxylation of butenyl and pentenyl glucosinolates in Brassica napus is investigated by studying the segregation of the degree of hydroxylation in seeds and leaves of recombinant lines derived from crosses between oilseed rape cultivars and synthetic B. napus lines. It is shown that two loci regulate hydroxylation in both leaves and seeds. Alleles at a locus on linkage group 13 (Gsl-oh-C) have a major effect while alleles at a homoeologous locus on group 3 ( Gsl-oh-A ) have a minor effect. The implication of these results for developing improved cultivars of oilseed rape is discussed.
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