Ghrelin-related peptides exert protective effects in the cerebral circulation of male mice through a nonclassical ghrelin receptor(s)

  • Ku J
  • Andrews Z
  • Barsby T
 et al. 
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Abstract

The ghrelin-related peptides, acylated ghrelin, des-acylated ghrelin, and obestatin, are novel gastrointestinal hormones. We firstly investigated whether the ghrelin gene, GOAT, and the ghrelin receptor (growth hormone secretagogue receptor 1a [GHSR1a]) are expressed in mouse cerebral arteries. Secondly, we assessed the cerebrovascular actions of ghrelin-related peptides by examining their effects on vasodilator nitric oxide (NO) and superoxide production. Using RT-PCR, we found the ghrelin gene and GOAT to be expressed at negligible levels in cerebral arteries from male wild-type mice. mRNA expression of GHSR1a was also found to be low in cerebral arteries, and GHSR protein was undetectable in GHSR-eGFP mice. We next found that exogenous acylated ghrelin had no effect on the tone of perfused cerebral arteries, or superoxide production. By contrast, exogenous des-acylated ghrelin or obestatin elicited powerful vasodilator responses (EC50 values

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Authors

  • Jacqueline M. Ku

  • Zane B. Andrews

  • Tom Barsby

  • Alex Reichenbach

  • Moyra B. Lemus

  • Grant R. Drummond

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