Gill histopathological changes in milkfish (Chanos chanos) exposed to acute toxicity of diesel oil

Abstract

In this study the 96-h LC 50 values of diesel oil and its effects on gill histopathology of milkfish (Chanos chanos) were determined. Young juvenile milkfish (W: 64±1.2gr, SL: 16.7±0.4Cm) were exposed to diesel oil at concentrations of 3.4, 3.9, 4.4, 5.5 and 7 ml/l. The experiments were performed as three replicates and all changes in the specimens were determined for each concentration. Water quality parameters of the test seawater were calculated. The 96-h LC50 value was found to be 5.12 ml/l in a static bioassay test system. The gill lamellae became lifted; secondary lamella fused and showed edema. Hyperplasia, hyperemia and hypertrophy of lamellar epithelial cells were distinct with mucus cells increasing. The result showed that acute oil toxicity severely affects on the mortality and gill structure which may be deleterious for milkfish populations. © IDOSI Publications, 2011.