Glial activation and segmental upregulation of interleukin-1beta (IL-1beta) in the rat spinal cord after surgical incision.

  • Fu D
  • Guo Q
  • Ai Y
 et al. 
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The present study investigated the expression patterns of glial cells and interleukin-1beta (IL-1beta) in the rat spinal cord after a surgical incision, which is closely related with clinical postoperative pain. Microglia and astrocytes became activated in the spinal cord following incision. Real-time polymerase chain reaction (PCR) and immunohistochemisty showed that IL-1beta mRNA and protein level in the spinal cord was transiently upregulated after surgical incision. The increased IL-1beta-immunoreactivity (IR) was mainly localized in neurons but not the activated microglia or astrocytes. Although obvious increase in IL-1beta-IR could be observed in the lumbar segments of the spinal cord ipsilateral to a hind paw incision, significant upregulation of IL-1beta was not detected in the lumbar segments following thoracic incision. The present study indicated that surgical incision could induce glial activation and segmental upregulation of IL-1beta in the spinal cord. The activated glial cells and upregulated IL-1beta, in turn, may be involved in the incision-induced pain hypersensitivity.

Author-supplied keywords

  • Animals
  • Disease Models, Animal
  • Immunohistochemistry
  • Interleukin-1
  • Interleukin-1: biosynthesis
  • Interleukin-1: genetics
  • Male
  • Neuroglia
  • Neuroglia: metabolism
  • Pain, Postoperative
  • Pain, Postoperative: etiology
  • Pain, Postoperative: metabolism
  • Polymerase Chain Reaction
  • RNA, Messenger
  • RNA, Messenger: biosynthesis
  • Rats
  • Rats, Wistar
  • Spinal Cord
  • Spinal Cord: metabolism
  • Thoracic Surgical Procedures
  • Thoracic Surgical Procedures: adverse effects
  • Up-Regulation

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  • Di Fu

  • Qulian Guo

  • Yuhang Ai

  • Hongwei Cai

  • Jianqin Yan

  • Ruping Dai

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