Global methods for protein glycosylation analysis by mass spectrometry.

  • Budnik B
  • Lee R
  • Steen J
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Abstract

Mass spectrometry has been an analytical tool of choice for glycosylation analysis of individual proteins. Over the last 5 years several previously and newly developed mass spectrometry methods have been extended to global glycoprotein studies. In this review we discuss the importance of these global studies and the advances that have been made in enrichment analyses and fragmentation methods. We also briefly describe relevant sample preparation methods that have been used for the analysis of a single glycoprotein that could be extrapolated to global studies. Finally this review covers aspects of improvements and advances on the instrument front which are important to future global glycoproteomic studies.

Author-supplied keywords

  • Acetylglucosamine
  • Acetylglucosamine: chemistry
  • Chromatography, Affinity
  • Chromatography, Affinity: methods
  • Electron Transport
  • Electrons
  • Gas Chromatography-Mass Spectrometry
  • Gas Chromatography-Mass Spectrometry: methods
  • Glycosylation
  • Lectins
  • Lectins: isolation & purification
  • Mass Spectrometry
  • Mass Spectrometry: methods
  • Protein Processing, Post-Translational

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Authors

  • Bogdan a Budnik

  • Richard S Lee

  • Judith a J Steen

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