GLUT4 ablation in mice results in redistribution of IRAP to the plasma membrane

  • Jiang H
  • Li J
  • Katz E
 et al. 
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Glucose transporter (GLUT) 4 is the insulin responsive glucose transporter in adipose tissue, skeletal muscle, and heart. Insulin elicits increased glucose uptake by recruiting GLUT4 from a specialized intracellular storage site to the cell surface. Expression of various proteins that colocalize with GLUT4 and/or are involved in insulin-stimulated GLUT4 translocation was examined in adipocytes as well as skeletal and cardiac muscles from GLUT4 null mice. Our data demonstrate that expression of insulin-regulated aminopeptidase (IRAP) is divergently regulated in GLUT4 null tissues, e.g., upregulated 1.6-fold in GLUT4 null adipocytes and downregulated in GLUT4 null skeletal muscle (40%) and heart (60%). IRAP exhibited abnormal subcellular distribution and impaired insulin-stimulated translocation in GLUT4-deficient tissues. We propose the compartment containing IRAP and proteins normally associated with GLUT4 vesicle traffics constitutively to the cell surface in GLUT4 null adipocytes and skeletal muscle. © 2001 Academic Press.

Author-supplied keywords

  • GLUT4 translocation
  • Insulin action
  • Insulin-regulated aminopeptidase
  • Vesicle trafficking

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  • Hua Jiang

  • Jing Li

  • Ellen B. Katz

  • Maureen J. Charron

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