Glycosylation Mutants of Cultured Cells

Abstract

Rapid progress in our understanding of glycosylation pathways in eukaryotes came with the application of genetic strategies to isolate mutants of mammalian cells and yeast with defects in glycan synthesis. This chapter reviews general methods used to isolate glycosylation mutants, the diversity of mutants that may be obtained, and the application of these mutants to address questions in glycobiology research. Many of the cell lines described in this chapter are available through the American Type Culture Collection. Glycosylation mutants of yeast are discussed in Chapter 21.