Gordonia malaquae sp. nov., isolated from sludge of a wastewater treatment plant

  • Yassin A
  • Shen F
  • Hupfer H
 et al. 
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Abstract

The taxonomic status of a bacterial isolate from the sludge of a wastewater treatment plant was characterized by using a polyphasic taxonomic approach. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV, short-chain mycolic acids that co-migrated with those extracted from members of the genus Gordonia, fatty acids C(16 : 0) and C(18 : 0) (found by pyrolysis gas chromatography) and a dihydrogenated menaquinone with nine isoprene units [MK-9(H2)] as the predominant menaquinone. The genus assignment was confirmed by 16S rRNA gene sequencing. Comparative analysis of the 16S rRNA gene sequence showed that the novel isolate constitutes a hitherto unknown subline within the genus Gordonia, displaying 95.9 to 97.6 % gene sequence similarity to the recognized species of the genus. The novel isolate was distinguished from the type strains of phylogenetically related species by using a set of phenotypic features. The genotypic and phenotypic data show that the new strain merits classification as a novel species of the genus Gordonia, for which the name Gordonia malaquae sp. nov. is proposed. The type strain is IMMIB WWCC-22(T) (=DSM 45064(T)=CCUG 53555(T)).

Author-supplied keywords

  • *Water Microbiology
  • Actinomycetales/chemistry/*classification/*isolati
  • Bacterial Typing Techniques
  • Carbon/metabolism
  • Cell Wall/chemistry
  • DNA, Bacterial/chemistry/genetics
  • DNA, Ribosomal/chemistry/genetics
  • Fatty Acids/analysis
  • Genes, rRNA
  • Molecular Sequence Data
  • Nitrogen/metabolism
  • Phylogeny
  • RNA, Bacterial/genetics
  • RNA, Ribosomal, 16S/genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Sewage/*microbiology
  • Vitamin K 2/analysis

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Authors

  • A F Yassin

  • F T Shen

  • H Hupfer

  • A B Arun

  • W A Lai

  • P D Rekha

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