Growing cholesterol-dependent NSO myeloma cell line in the wave bioreactor system: Overcoming cholesterol-polymer interaction by using pretreated polymer or inert fluorinated ethylene propylene

  • Kadarusman J
  • Bhatia R
  • McLaughlin J
 et al. 
  • 30


    Mendeley users who have this article in their library.
  • 23


    Citations of this article.


Difficulty in growing cholesterol-dependent NS0 cells in the Wave bioreactor using the original low-density polypropylene (LDPE) bags has been encountered. It has been shown that in these bags chemically defined cholesterol is depleted from solution and therefore unavailable for the cells. Our data suggest that the cause of the depletion is not chemical but is due to the physical structure of the polymer. It is proposed that polymer structures with inkbottle pores retain cholesterol, whereas structures with V-shaped pores adsorb cholesterol reversibly. Ultra-low-density polyethylene (ULDPE) bags can support cell growth but need to be pretreated with excess cholesterol. Another material, fluorinated ethylene propylene (FEP) does not need to be pretreated and is found to be superior (negligible cholesterol adsorption) as a result of its inert characteristics.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Judith Kadarusman

  • Ravi Bhatia

  • John McLaughlin

  • Weng Long R. Lin

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free