GTP, a nonsubstrate of ATP citrate lyase, is a phosphodonor for the enzyme histidine autophosphorylation

  • Tuháčková Z
  • Křivánek J
  • 6

    Readers

    Mendeley users who have this article in their library.
  • 10

    Citations

    Citations of this article.

Abstract

ATP citrate lyase (EC 4.3.1.8.) was shown to be a major phosphorylated protein of a fraction derived from Zajdela rat hepatoma by chromatography on heparin-Ultrogel, after the incubation with [γ-32P]ATP or [γ-32P]GTP. Histidine was the only amino acid in the purified enzyme phosphorylated by [γ-32P]ATP or [γ-32P]GTP in the autocatalytic reaction which occurred apparently through an intramolecular mechanism regardless of a donor of phosphate. GTP inhibits the ATP-dependent autophosphorylation competitively despite its failure to replace ATP in the formation of acetyl-CoA catalyzed by this enzyme.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free