High-risk human papillomavirus E7 oncoprotein detection in cervical squamous cell carcinoma.

  • Ressler S
  • Scheiden R
  • Dreier K
 et al. 
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PURPOSE: Persistent infections by high-risk human papillomavirus (HPV) types are the main etiologic factor for cervical cancer. The objective of this study was to evaluate whether high-risk E7 oncoprotein is adequate as a marker for the detection of cervical cancer.

EXPERIMENTAL DESIGN: HPV typing was done in biopsies from 58 cervical carcinoma and 22 normal cervical squamous epithelia. The HPV-16 E7, HPV-18 E7, and HPV-45 E7 oncoprotein levels were monitored by immunohistochemistry and compared with those of p16(INK4a) and Ki67.

RESULTS: Fifty-five (94.8%) tumors were high-risk HPV-DNA-positive (46 HPV-16, 2 HPV-16 and HPV-18, 4 HPV-18, 1 HPV-33, and 2 HPV-45). HPV-DNA could not be detected in three tumors (5.2%). High HPV E7 oncoprotein levels were shown in 57 cervical cancers (98.3%), without correlation between expression levels and tumor stages.

CONCLUSION: This is the first study which systematically analyzes the levels of the major HPV oncoproteins in cervical carcinomas demonstrating that the high-risk HPV E7 proteins are regularly expressed in these cancers. This suggests that high-risk E7 oncoproteins are necessary for cervical cancers and apparently essential as tumor marker.

Author-supplied keywords

  • Adult
  • Aged
  • Animals
  • Antibodies, Viral
  • Antibodies, Viral: chemistry
  • Antibodies, Viral: immunology
  • Carcinoma, Squamous Cell
  • Carcinoma, Squamous Cell: metabolism
  • Carcinoma, Squamous Cell: pathology
  • Carcinoma, Squamous Cell: virology
  • Cell Cycle
  • Cell Cycle: physiology
  • Cyclin-Dependent Kinase Inhibitor p16
  • Cyclin-Dependent Kinase Inhibitor p16: metabolism
  • DNA, Viral
  • DNA, Viral: analysis
  • Female
  • Humans
  • Immunohistochemistry
  • Ki-67 Antigen
  • Ki-67 Antigen: metabolism
  • Mice
  • Middle Aged
  • NIH 3T3 Cells
  • Neoplasm Staging
  • Papillomaviridae
  • Papillomaviridae: genetics
  • Papillomaviridae: isolation & purification
  • Papillomavirus E7 Proteins
  • Papillomavirus E7 Proteins: analysis
  • Papillomavirus E7 Proteins: biosynthesis
  • Papillomavirus E7 Proteins: genetics
  • Papillomavirus Infections
  • Papillomavirus Infections: complications
  • Papillomavirus Infections: metabolism
  • Papillomavirus Infections: virology
  • Polymerase Chain Reaction
  • Tumor Markers, Biological
  • Tumor Markers, Biological: analysis
  • Tumor Markers, Biological: biosynthesis
  • Tumor Markers, Biological: genetics
  • Uterine Cervical Neoplasms
  • Uterine Cervical Neoplasms: metabolism
  • Uterine Cervical Neoplasms: pathology
  • Uterine Cervical Neoplasms: virology

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  • Sigrun Ressler

  • René Scheiden

  • Kerstin Dreier

  • Andreas Laich

  • Elisabeth Müller-Holzner

  • Haymo Pircher

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