High-throughput screening approaches to identify regulators of mammalian autophagy

  • Joachim J
  • Jiang M
  • McKnight N
 et al. 
  • 39


    Mendeley users who have this article in their library.
  • 9


    Citations of this article.


This article discusses the issues to consider in the development and implementation of high-throughput screens (HTSs) using both siRNA libraries and small molecule compound collections, in order to discover autophagy regulators in mammalian cells. We discuss how to develop the screen, focusing on the key parameters to establish in order to perform a successful screen. As our understanding of autophagy increases and its impact on human disease is elucidated, this technology can be further exploited to uncover novel genes, which may one day become new therapeutic targets.

Author-supplied keywords

  • Amino acid starvation
  • Autophagosome
  • Autophagy
  • Drug and compound libraries
  • GFP-LC3
  • High-throughput screens
  • P62
  • SiRNA
  • Z-score

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free