α-Amylase gene expression was detected in newly hatched seabass (Lates calcarifer) larvae and peaked at around first feeding. This suggests a greater importance of carbohydrates during early larval development than might be expected for carnivorous fishes. In vivo cortisol and triiodothyronine (T3) treatment of seabass larvae upregulated α-amylase gene expression. The identification of a functional glucocorticoid-response element (GRE) on the amylase gene promoter indicates that cortisol (glucocorticoid) stimulation of amylase gene expression is direct via GRE. However no TRE (thyroid-response element) was found on the amylase gene and its promoter, and various concentrations of T3 (1nM-10μM) also did not induce α-amylase gene promoter activity in rat AR42-J cells transfected with the promoter construct, unlike dexamethasone treatment. This suggests that T 3 stimulation of amylase gene expression in vivo was indirect, probably secondary to its promotion of one or more developmental processes. © 2004 Elsevier Inc. All rights reserved.
CITATION STYLE
Ma, P., Reddy, K. P., Chan, W. K., & Lam, T. J. (2004). Hormonal influence on amylase gene expression during Seabass (Lates calcarifer) larval development. General and Comparative Endocrinology, 138(1), 14–19. https://doi.org/10.1016/j.ygcen.2004.04.007
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