Identification of complex acyl lipids ideally includes the normal-phase HPLC to separate the acyl lipid classes followed by reversed-phase HPLC to separate the molecular species of a lipid class. Both polar lipid classes and non-polar lipid classes have been separated by normal-phase HPLC, mostly on silica and diol columns using binary gradients. The tentative identification of lipid classes can be made by HPLC retention times and the co-chromatography with lipid class standards. Various normal-phase HPLC systems separating lipid classes are reviewed here.
CITATION STYLE
Lin, J. T. (2007, January). HPLC separation of acyl lipid classes. Journal of Liquid Chromatography and Related Technologies. https://doi.org/10.1080/10826070701435020
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