HPLC-MS technique for radiopharmaceuticals analysis and quality control.

  • Macasek F
  • Buriova E
  • Bruder P
 et al. 
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Potentialities of liq. chromatog. with mass spectrometric detector (MSD) were investigated with the objective of quality control of radiopharmaceuticals, 2-deoxy-2-[18F]fluoro-D-glucose (FDG) being an example. Screening of suitable MSD anal. lines is presented. Mass spectrometric monitoring of acetonitrile-aq. ammonium formate eluant by neg. charged FDG. HCO2- ions enables isotope anal. (specific activity) of the radiopharmaceutical at m/z 227 and 226. Kryptofix 222 provides an intense MSD signal of the pos. ion assocd. with NH4+ at m/z 394. Expired FDG injection samples contain decompn. products from which at least one labeled by 18F and characterized by signal of neg. ions at m/z 207 does not correspond to FDG fragments but to CS decompn. products. A glucose chromatog. peak, characterized by m/z 225 neg. ion is accompanied by a tail of a component giving a signal of m/z 227, which can belong to [18O]glucose; isobaric sorbitol signals were excluded but FDG-glucose assocn. occurs in the co-elution of sepn. of model mixts. The latter can actually lead to a convoluted chromatog. peak, but the absence of 18F makes this inconsistent. Quantification and validation of the FDG component anal. is under way. [on SciFinder(R)]

Author-supplied keywords

  • HPLC MS radiopharmaceutical
  • deoxyfluoroglucose HPLC MS quality control

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  • F Macasek

  • E Buriova

  • P Bruder

  • H Vera-Ruiz

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